Analysis of dairy products and food products

Analysis of dairy products and food products

Analysis of dairy and food products is essential to evaluate their conformity to specification & regulations and deceleration of nutritional information. In addition, chemical analysis of fats and oil is also taken up.

a)  Laboratory analyses various dairy products as per Indian Standards for chemical and microbiological parameters. The following milk products are being tested currently by the laboratory.

1.    Milk Powder – IS 1165:2002 with Amendment No. 1
2.    Processed Cheese and Processed Cheese Spread - IS 2785:1979
3.    Shrikhand – IS 9532:1980
4.    Paneer – IS 10484:1983
5.    Skimmed Milk Powder – IS 13334 (Part 1):2014
6.    Skimmed Milk Powder (Extra Grade) – IS 13334 (part 2):1992 with Amendment No. 3
7.    Pasteurized Butter – IS 13690:1992 with Amendment No. 1

b)  Laboratory analyses wide variety of parameters required as nutritional information by the Dairy and food Industry.

Group of Analysis

Parameters

Equipment/Technique

Compositional parameters

Protein,  Fat, Carbohydrate,  Total solids,   Sugar

 BIS/AOAC method

Fatty Acids/Cholesterol

Fatty Acids profiles, Saturated Fat, Unsaturated Fat, Poly unsaturated fat

Gas chromatograph with FID detector

Sugar Profile

Lactose, Sucrose, Glucose

HPLC with RI detector.

Vitamins

Vitamin A, D,  E,  and Beta carotene, Vitamin C

HPLC with PDA detector, Preparative HPLC

Thiamine, Riboflavin, Niacin, Pantothenic
Acid, Vitamin B12, Folic acid and Biotin

Microbiological method based upon micro titre plate test.

Minerals

Calcium, Phosphorus Sodium, Potassium, Magnesium etc.

ICP-OES with microwave digestion system.

c)  Laboratory analyses wide variety of contaminant, toxicant and safety parameters in milk and food products.

Group of Analysis

Parameters

Equipment/Technique

Drug residues

Albendazole, Ivermectin, Beta lactam

HPLC with PDA & Fluorescence detectors and Delvo kit.

Contaminants

Melamine

HPLC with PDA

Mycotoxin

Aflatoxin M1,  B1, G1, B2 and G2

HPLC with fluorescence & Kobra cell and ELISA based assay.

Heavy metals

Arsenic, Cadmium, Lead,  Tin

ICPMS- with microwave digestion system.

Pesticides

Organo chloro and Organo phosphorus group.

Gas chromatograph with ECD and FPD detector.

Microbiological parameters

Coliform count, Total Plate , count, Yeast & Mould count, Thermophillic count, Lactic Acid Bacteria count,   

Enumeration of E.coli,

Detection of E. Coli.

Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogens and Clostridium perfringens.

Bio safety cabinet, Laminar air flow bench, Autoclave, Incubators Colony counters.

Adulterants

Neutralizer, Formalin, Starch, Urea, Detergent, added glucose, Salicylic acid, Boric acid and borate, Nitrate, Sodium chloride and cane sugar.

By chemical analysis.